Platelets are small, sticky cell fragments that circulate in blood and can adhere to other sticky blood cells, such as monocytes. Because the resulting cell clumps can interfere with certain cell isolations, it is often necessary to remove platelets from human blood samples prior to cell separation. This video demonstrates how to use a slow spin to reduce platelet contamination in fresh human blood samples after density gradient centrifugation. Pipette off and discard most of the yellow-colored platelet-enriched plasma layer before isolating the mononuclear cells. Please see your density gradient medium product information sheet or our technical video for more details on this procedure. Wash the collected cells in PBS containing 2% FBS. Centrifuge the sample for 10 minutes at 120 x g with the brake off.
Carefully remove and discard the supernatant. Repeat the wash process 2 more times. Your purified sample is now ready for cell isolation and downstream applications. We hope that you have found this video on how to use a slow spin to reduce platelet contamination in fresh human blood samples helpful. For more technical tips, including videos covering other sample preparation procedures, please visit our website at stemcell.com. .